To catalogue
OYC Americas Enloase
Enloase is Sourced from Yeast in 50% Glycerol solution form.
Properties
- Pharma and Nutraceuticals Functions
- Enzyme
Product families
Applications
Healthcare & Pharma — Medical
- Laboratory & Diagnostics
Product Families
Pharmaceuticals & Nutraceuticals — Excipients
- Formulation & Processing Aids
Other characteristics
- Functions
- Enzyme
- Markets
- Healthcare & Pharma
- PACKAGING
- 50 ku, 250 ku, 1000 ku
- Preparation
- Product Code：Enolase-05 50% Glycerol solution
- IU per 1 ml solution is approximately 600 units.
- Technology
- Pharmaceuticals & Nutraceuticals
- Packaging Type
- Bulk
- Assay Procedure
Spectrophotometric Method; 2.31 mL Triethanolamine-HCl-NaOH buffer （0.1 mol/L, pH 7.5）; 0.15 mL MgCl2（0.1 mol/L）; 0.15 mL KCl（2.0 mol/L）; 0.15 mL ADP（10 mmol/L）; 0.09 mL 2,3-Diphospho-D-glycerate（10 mmol/L）; 0.15 mL Glycerate-3-phosphate（0.4 mol/L）; 0.05 mL NADH（10 mg/mL）dissoluted in Tris （10 mmol/L）; 0.002 mL PK（5,000 IU/mL）; 0.003 mL LDH（10,000 IU/mL）; 0.01 mL （r）PGAM（770 IU/mL）; 0.02 mL Enolase（about 3 IU/mL）
- Equation
2-Phospho-D-glycerate ＝ Phosphoenolpyruvate + H2O
Technical data
Table 1 of 2
2 rows| Characteristic | Value | Unit |
|---|---|---|
| Specific Activity (at 25C° and pH7.5) | 40.0 | IU/mg protein |
| Specific Activity | min. 40 | IU/mg protein |
Table 2 of 2
2 rows| Characteristic | Value | Unit |
|---|---|---|
| Phosphoglyceromutase | max. 0.0002 | — |
| Pyruvate kinase | max. 0.02 | % |
