To catalogue
OYC Americas Bacterial Protocatechuate 3,4-Dioxygenase (rPCO)
Bacterial Protocatechuate 3,4-Dioxygenase (rPCO) is sourced fron Recombinant from Bacteria in solution form.
Properties
- Physical Form
- Liquid
- Pharma and Nutraceuticals Functions
- Enzyme
Product families
Applications
Healthcare & Pharma — Medical
- Laboratory & Diagnostics
Product Families
Pharmaceuticals & Nutraceuticals — Excipients
- Formulation & Processing Aids
Other characteristics
- Functions
- Enzyme
- Markets
- Healthcare & Pharma
- Preparation
- Product Code：rPCO-04 Solution
- IU per 1 ml solution is ＞80 units
- Technology
- Pharmaceuticals & Nutraceuticals
- Packaging Type
- Bulk
- Assay Procedure
- Spectrophotometric Method
- Wavelength；290 nm
- Light path length；1 cm
- Temperature；37℃
- Pipette the following reagents into a cuvette
- Product Features
Gene: Protocatechuate 3,4-dioxygenase gene cloned from Acinetobacter baumannii Production: Purified from the extract of transformed E.coli by conventional chromatographies Purity: Single major band on SDS-PAGE (low contaminants from host E.coli) Application: Useful for removal of protocatechuate derived from choline esterase determination Stability: Wide range of pH stability from 5 to 10
- Equation
Protocatechuate + O2 ＝ 3-Carboxy-cis,cis-muconate
- Storage Condition
- 1 - 10°C (do not freeze)
Technical data
Table 1 of 2
1 row| Characteristic | Value | Unit |
|---|---|---|
| Specific Activity | 3.0 | IU/mg protein |
Table 2 of 2
9 rows| Characteristic | Value | Unit |
|---|---|---|
| NADH oxidase | max. 0.01 | % |
| Alkaline phosphatase | max. 0.002 | % |
| pH stability | pH 5.0 - 10.5（11, 1 week） | — |
| Thermal stability （pH 8.0, 15 min） | max. 60 | ℃ |
| Optimum pH | 9.0 | — |
| Optimum temperature | 65.0 | ℃ |
| Km value | 28（Protocatechuate） | μmol/L |
| Molecular Weight (SDS-PAGE, α subuit） | 28.0 | kD |
| Molecular Weight (SDS-PAGE, β subunit） | 24.0 | kD |
