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OYC Americas Hexokinase, recombinant from Kluyveromyces fragilis (rHK)

Hexokinase, recombinant from Kluyveromyces fragilis (rHK) is Sourced from Kluyveromyces fragilis lyophilized powder form.

Properties

Physical Form
  • Powder
  • Solid
Pharma and Nutraceuticals Functions
Enzyme

Product families

Applications

Healthcare & Pharma — Medical
  • Laboratory & Diagnostics

Product Families

Pharmaceuticals & Nutraceuticals — Excipients
  • Formulation & Processing Aids

Other characteristics

Functions
Enzyme
Markets
Healthcare & Pharma
PACKAGING
1,000 units
Preparation

Product Code:rHK-93 Lyophilized powder (contains no ammonium sulfate)

Technology
Pharmaceuticals & Nutraceuticals
Assay Procedure
  • Spectrophotometric Method
  • 3.00 ml 40 mg/ml Glucose, 10 mmol/L MgCl₂
  • 0.5 mmol/L ATP, 0.5 mmol/L NADP⁺
  • 5.5 U/ml G-6-PDH, 80 mmol/L
  • Triethanolamine(TEA)- HCl buffer (pH 7.5, 25゚C)
  • 0.02 ml rHK(about 2 U/ml)
Equation
ATP + D-Hexose = ADP + D-Hexose 6-phosphate
Storage Condition
Below -20°C

Technical data

Table 1 of 2

2 rows
CharacteristicValueUnit
Specific Activity150.0IU/mg protein
Specific Activitymin. 150IU/mg protein

Table 2 of 2

19 rows
CharacteristicValueUnit
Phosphoglucose isomerasemax. 0.00003%
Glutathione reductasemax. 0.005%
Myokinasemax. 0.001%
Phosphogluconate dehydrogenasemax. 0.00001%
Phosphoglucomutasemax. 0.001—
Glucose-6-phosphate dehydrogenasemax. 0.005%
Creatine kinasemax. 0.005%
ATPasemax. 0.003%
Glucose dehydrogenasemax. 0.001%
Invertasemax. 0.00005—
Alkaline phosphatasemax. 0.00001%
pH stabilitypH 6.0 - 9.0(4, 1 week)—

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